Fish Melatonin (MT) ELISA Kit
Species Reactivity : Fish
UniProt : N/A
Abbreviation : MT
Alternative Names : N-Acetyl-5-Methoxytryptamine
Application : ELISA
Range : 12.35-1000 pg/mL
Sensitivity : 4.94 pg/mL
Intra-AssayCV : ?4.1%
Inter-AssayCV : ?7.8%
Recovery : 0.97
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate MT in samples. An antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for MT is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Melatonin, 5-methoxy-N-acetyltryptamine, is a hormone found in all living creatures from algae to humans, at levels that vary in a daily cycle. Many biological effects of melatonin are produced thro µgh activation of melatonin receptors,while others are due to its role as a pervasive and extremely powerf µL antioxidant with a partic µLar role in the protection of nuclear and mitochondrial DNA.Melatonin is secreted by the pineal gland in the brain and is important in the reg µLation of many hormones in the body. Among its key roles, melatonin controls the body's circadian rhythm, an internal 24-hour time-keeping system that plays an important role in when fall asleep and when wake up. Darkness stim µLates the release of melatonin and light suppresses its activity.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).