Flag Tag Monoclonal Antibody

Volume : 50 µL
Clone Number : 4G5C8
Aliases : DDK tag, DYKDDDDK, Flag, Flag Tag
Product Type : Tag/Control Antibodies
Immunogen Species :
UniProt ID :
Immunogen : DYKDDDDK(Flag) synthetic peptide conj µgate to KLH
Raised in : Mouse
Species Reactivity :
Tested Applications : ELISA, WB, IF, IP; Recommended dilution : WB : 1 : 1000-1 : 10000, IF : 1 : 100-1 : 300, IP : 0.5µl-2µl
Background : Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with m µLtiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cell µLar localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Clonality : Monoclonal
Isotype : IgG2a
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : Flag Tag
Research Areas :