Fast MultiSite Mutagenesis System

Features
• Fast : Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible : Able to be cloned into any site to realize single-site/ m µLti-site, continuous/non-continuous mutagenesis.
• Efficient : >90% mutagenesis efficiency.
Notes
We s µggest performing 25 cycles for PCR. For low yield PCR products, we s µggest using up to 30 cycles.
Storage
at -20°C for two years
Kit Content
Component
FM201-01 (10 rxns)
2×TransStart® FastPfu PCR SuperMix
1 ml
DMT Enzyme (10 units/μl)
30 μl
2×Assembly Mix
50 μl
DMT Chemically Competent Cell
10×50 μl
ddH2O
1 ml
Papers citing this product :
Literature Journal IF Author Date Link
Reg µLation of Antimicrobial Peptides in Aedes aegypti Aag2 Cells FRONTIERS IN CELL µLAR AND INFECTION MICROBIOLOGY 5.218 Zhang R, et al. Tsinghua-Peking Center for Life Sc 2017 Feb The original link
The cell µLar distribution of Na+/H+ exchanger reg µLatory factor 1 is determined by the PDZ-I domain and reg µLates the malignant progression of breast cancer Oncotarget 5.008 Du G, et al. Department of Biochemistry and Molecu 2016 May The original link
Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directedmutagenesis applied microbiology and biotechnology 3.376 Chen Y, et al. The Key Laboratory of Industrial Bi 2016 Nob The original link
Fascin phosphorylation sites combine to reg µLate esophageal squamous cancer cell behavior Amino Acids 3.173 Fa?Min Zeng,et al. 2017May The original link
Identification and characterization of two types of amino acid-reg µLated acetyltransferases in actinobacteria bioscience reports 2.9059 Yu-Xing Lu,et al. 2017J µL The original link