FlyCut® HindIII

Product Details
FlyCut® HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. The molec µLar weight is 35.8kDa, with the recognition site at A^AGCTT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...A↓AGCTT...3’
3’...TTCGA↑A...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JH101-01 JH101-02
FlyCut® HindIII 5,000 units 2×5,000 units
10×FlyCut® Buffer 2×1 ml 3×1 ml
10×DNA Loading Buffer 1 ml 1 ml