FlyCut® NheI
Product Details
FlyCut® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molec µLar weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...G↓CTAGC...3’
3’...CGATC↑G...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Product Contents
Component JN301-01 JN301-02
FlyCut® NheI 500 units 2×500 units
10×FlyCut® Buffer 250 μl 250 μl
10×DNA Loading Buffer 1 ml 1 ml