FlyCut® SacII

Product Details
FlyCut® SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molec µLar weight is 32.1 kDa, with the recognition site at CCGC^GG The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

Recognition Site
5’...CCGC↓GG...3’
3’...GG↑CGCC...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JS201-01 JS201-02
FlyCut® SacII 1000 units 2×1000 units
10×FlyCut® Buffer 250 μl 500 μl
10×DNA Loading Buffer 1 ml 1 ml