FlyCut® SpeI

Product Details
FlyCut® SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molec µLar weight is 21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...A↓CTAGT...3’
3’...TGATC↑A...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JS601-01 JS601-02
FlyCut® SpeI 250 units 2×250 units
10×FlyCut® Buffer 250 μl 250 μl
10×DNA Loading Buffer 1 ml 1 ml