Fish Immunoglobulin M (IgM) ELISA Kit

Species Reactivity : Fish
UniProt : N/A
Abbreviation : IgM
Alternative Names : N/A
Application : ELISA
Range : 1.56-100 ?g/mL
Sensitivity : 0.75 ?g/mL
Intra-AssayCV : ?6.8%
Inter-AssayCV : ?8.8%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate IgM in samples. An antibody specific for IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIgM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for IgM is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview :
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