Fish Triglyceride (TG) ELISA Kit

Species Reactivity : Fish
UniProt : N/A
Abbreviation : TG
Alternative Names : N/A
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.7%
Inter-AssayCV : ?7.9%
Recovery : 1.01
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TG in samples. An antibody specific for TG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TG is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Thyroglob µLin (Tg) is a 660 kDa, dimeric protein produced by and used entirely within the thyroid gland. In earlier literature, Tg was referred to as "colloid".Thyroglob µLin sho µLd not be confused with Thyroxine-binding glob µLin, a carrier protein responsible for carrying the thyroid hormones in the blood.Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). The active form of triiodothyronine, 3, 5, 3' triiodothyronine, is produced both within the thyroid gland and periphery by 5'-deiodinase (which has been referred to as tetraiodothyronine 5' deiodinase.)Thyroglob µLin levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. Thyroglob µLin levels in the blood can also be elevated in cases of Graves' disease.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).