FlyCut® EagI

Product Details
FlyCut® EagI is expressed and purified from E.coli that carries the recombinant EagI gene. The molec µLar weight is 35.1 kDa, with the recognition site at C^GGCCG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...C↓GGCCG...3’
3’...GCCGG↑C...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JE101-01 JE101-02
FlyCut® EagI 250 units 2×250 units
10×FlyCut® Buffer 250 μl 250 μl
10×DNA Loading Buffer 1 ml 1 ml