FlyCut® EcoRI

Product Details
FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molec µLar weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...G↓AATTC...3’
3’...CTTAA↑G...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JE201-01 JE201-02
FlyCut® EcoRI 5,000 units 2×5,000 units
10×FlyCut® Buffer 2×1 ml 3×1 ml
10×DNA Loading Buffer 1 ml 1 ml