FlyCut® PstI
Product Details
FlyCut® PstI is expressed and purified from E.coli that carries the recombinant PstI gene. The molec µLar weight is 38.3 kDa, with the recognition site at CTGCA^G. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...CTGCA↑G...3’
3’...G↑ACGTC...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Product Contents
Component JP101-01 JP101-02
FlyCut® PstI 5,000 units 2×5,000 units
10×FlyCut® Buffer 2×1 ml 3×1 ml
10×DNA Loading Buffer 1 ml 1 ml