FlyCut® SacI

Product Details
FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molec µLar weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...GAGCT↓C...3’
3’...C↑TCGAG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JS101-01 JS101-02
FlyCut® SacI 1000 units 2×1000 units
10×FlyCut® Buffer 250 μl 500 μl
10×DNA Loading Buffer 1 ml 1 ml