FlyCut® SmaI

Product Details
FlyCut® SmaI is expressed and purified from E.coli that carries the recombinant SmaI gene. The molec µLar weight is 29.7 kDa, with the recognition site at CCC^GGG The reaction is conducted for 5-15 minutes at 25oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

Recognition Site
5’...CCC↓GGG...3’
3’...GGG↑CCC...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JS501-01 JS501-02
FlyCut® SmaI 1000 units 2×1000 units
10×FlyCut® Buffer 250 μl 500 μl
10×DNA Loading Buffer 1 ml 1 ml