FlyCut® SphI

Product Details
FlyCut® SphI is expressed and purified from E.coli that carries the recombinant SphI gene. The molec µLar weight is 26.8 kDa, with the recognition site at GCATG^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...GCATG↓C...3’
3’...C↑GTACG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JS701-01 JS701-02
FlyCut® SphI 250 units 2×250 units
10×FlyCut® Buffer 250 μl 250 μl
10×DNA Loading Buffer 1 ml 1 ml